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Information × Registration Number 0220U001166, 0115U004198 , R & D reports Title Improvement of gene delivery efficiency into plants by using novel nanoscale polycationic polymers popup.stage_title Head Stoika Rostyslav, Registration Date 30-01-2020 Organization Institute of cell biology, National Academy of Sciences of Ukraine popup.description2 We have explored the possibility of application of DMAEMA-containing polymers that were synthesized at the Department of Organic Chemistry of the National University "Lviv Polytechnic" for the delivery of foreign genetic material into cells of dicotyledonous plants (tomato Lycopersicon esculentum) and protoplasts of moss Ceratodon urpureus. The PCR method confirmed the integration of the lactoferrin hLF gene into 2 lines of Lycopersicon esculentum tomato of Lagidny variety, transformed in the presence of polymers TN 83/6 and TN 84/5. PCR method confirmed that genetic transformants of moss Ceratodon purpureus contain the construct of of green fluorescent protein gene under it delivery to the protoplasts by polymers TN 83/5, TN 83/6, BG-2m, LAcr-DM2, LAcr-DM1, BAcr-DM2, BAcr -DM1 and carrier F8-DM2. Thus, the method of transformation of plant cells using polymers based on DMAEMA is a promising for the genetic transformation of plants of different systematic groups. Product Description popup.authors Ключівська Ольга Юріївна Фінюк Наталія Степанівна popup.nrat_date 2020-04-02 Close