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Information × Registration Number 0220U001348, 0119U002307 , R & D reports Title Immunogenetics of malignant growth: the effect of Bcl-2 gene expression on the state of antitumor immunity and with the corrective influence of nanomaterials popup.stage_title Head Lisnychuk Natalia Yevgenivna, Кандидат біологічних наук Registration Date 10-03-2020 Organization Higher State Educational Establishment I.Ya. Horbachevsky Ternopil State Medical University popup.description2 GDR Report: 70 pages, 30 figures, 7 tables, 74 sources. Induced carcinogenesis, spleen, immune system, morphology. Object of the study - immunological and metabolic mechanisms of oncogenesis and structural changes of organs of lymphoid system of animals with simulated colon adenocarcinoma. Aim of the study: To find out the peculiarities of the development of metabolic and immunoreactive disorders in experimental oncogenesis. Research methods: experimental - modeling of chemically induced carcinogenesis in outbred white rats with development of colon adenocarcinoma in situ; morphological - the study of the patterns of structural organization of organs of the lymphoid system, in particular, the spleen, at the microscopic and submicroscopic levels; immunological - determination of serum immunoglobulin A, M, G content, circulating immune complexes, concentrations of IL-1?, IL-6, IL-2, IL-4, IL-10, TNF?; biochemical studies of endogenous intoxication indicators, activities of oxidative stress factors, enzymatic and glutathione-dependent units of the antioxidant system; statistical. Under conditions of experimental carcinogenesis, significant metabolic disorders develop in the test animals, which are exacerbated by the manifestation of neoplastic endotoxemia syndrome; development of oxidative stress and imbalance of functioning of enzymatic and glutathione-dependent antioxidant protection units. Changes in immunological reactivity are accompanied by increasing concentrations of Ig A, Ig M, Ig G, and a significant accumulation of circulating immune complexes. Significant changes of the cytokine matrix were established, which was shown by a significant increase in the concentrations of IL-4, IL-6, IL-10, TNF? with a significant decrease in the content of IL-2. Histological studies of the spleen of animals in the dynamics of the formation of chronic neoplastic intoxication induced by the introduction of dimethylhydrazine revealed the development of destructive-degenerative changes of vessels, stroma, red and white pulp of the spleen, the severity of which increased in accordance with the observation period. Product Description popup.authors Калінічук Любов Олександрівна Крамар Соломія Богданівна Лісничук Наталя Євгенівна Небесна Зоя Михайлівна Олещук Олександра Михайлівна Сорока Юрій Вікторович Стравський Ярослав Степанович Яворська Світлана Іванівна popup.nrat_date 2020-07-03 Close