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Information × Registration Number 0220U100210, 0119U102364 , R & D reports Title Investigation of the effect of genes of desaturase cyanobacteria in transgenic tobacco on the adaptation of plants to ionizing and ultraviolet radiation. popup.stage_title Head Kyrpa-Nesmiian Tetiana M., Registration Date 13-01-2020 Organization Institute of Cell Biology and Genetic Engineering popup.description2  Used tobacco plants Nicotiana tabacum containing in their genome gene Δ9-acyl-lipid desaturase cyanobacteria Synechococcus vulcanus or gene Δ12-acyl-lipid desaturase cyanobacteria Synechocystis sp. PCC 6803 in work. Wild-type N.tabacum tobacco and N.tabacum tobacco containing the genome and expressing the gfp:licBM3 reporter gene were used as controls. The researchers found an increase in the proportion of linoleic acid in plants expressing the desA gene and linolenic in plants expressing the desC gene compared to control plants. The effect of ionizing radiation on plants in doses of 1Gy, 5Gy, 10Gy was investigated on the device RUM-17. They found an increase in SOD activity, investigated a decrease in MDA, a lower level of electrolyte loss in plants with desaturase genes compared to control plants. Analysis of plant resistance to ultraviolet radiation was carried out after exposure to the dominant spectrum C OSRAM lamp at doses of 1308 J/m² and 2616 J/m². The analysis of SOD activity revealed no statistically significant differences between the studied and control plants, found an increase in flavonoids after exposure to UV at a dose of 2616 J/m² in all transgenic plants, including transgenic control. A study of MDA showed that it was lower in plants that contain in their genome and express genes of cyanobacterium desaturases after exposure to UV at doses of 1308 J/m² and 2616 J/m² compared to control plants. Investigating the effect of desaturase gene expression on tobacco on resistance to UV and IR, they found a general tendency to decrease MDA in plants containing their genome and expressing desaturase cyanobacterial genes, which may indicate less oxidative degradation of unsaturated FA. Product Description popup.authors Potrokhov Andrii O. popup.nrat_date 2020-04-02 Close