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Information × Registration Number 0220U100333, 0119U102416 , R & D reports Title The functional activity of phagocytic cells as a predictor of the effectiveness of doxorubicin antitumor action popup.stage_title Head Prokhorova Iryna V., Кандидат біологічних наук Registration Date 15-01-2020 Organization R.E. Kavetsky Institute of Experimental Pathology, Oncology and Radiobiology National Academy of Sciences of Ukraine popup.description2  Object of study – parental and doxorubicin-resistant strains of Walker-256 carcinosarcoma. The aim of this work is an experimental study of the functional activity of phagocytic blood cells and peritoneal cavity cells of rats with the transplanted Walker carcinosarcoma as potential markers of the sensitivity of this tumor to doxorubicin treatment. Methods – methods of experimental oncology, flow cytometry, hematological and statistical methods. Hematological indices and reactive oxygen and nitrogen (ROS) species production by neutrophils and peripheral blood monocytes (percentage of ROS-generating cells and intensity of ROS production) of intact rats and rats with parental and resistant carcinosarcoma strains were investigated. The study has shown that the growth of two variants of Walker carcinosarcoma with different sensitivity to doxorubicin is accompanied by a significant increase in the absolute amount of neutrophils in the peripheral blood of animals with tumors, more pronounced for the resistant variant. Also, the level of ROS in neutrophils and blood monocytes of rats with the resistant variant of Walker carcinosarcoma was greatly and statistically significantly higher than the corresponding indexes in rats with the sensitivity variant of the tumor and intact animals, indicating the possibility of using these indices to monitor changes of the tumor to doxorubicin treatment. Product Description popup.authors Ivanchenko Alina V. Prokhorova Iryna V. popup.nrat_date 2020-04-02 Close