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Information × Registration Number 0220U100868, 0119U101455 , R & D reports Title Ion and membrane mechanisms of regulation of calcium homeostasis and myometrial contraction activity at different stages of pregnancy popup.stage_title Head Kosterin Sergiy O., Доктор біологічних наук Registration Date 29-01-2020 Organization Department for special training National academy of science of Ukraine at Kiev University popup.description2 Using in vitro tensiometry of the isolated myometrium strips of TRPV4 agonists and antagonists were shown to develop different effects in rat and human tissues in normal and pathophysiological condition as well as we observed that in the myometrium response to oxytocin TRPV4 play a role of the limiting factor. In this research new evidences about mechano-sensitive properties of TRPC4 were obtained: the decrease of the amplitude of contractile force and duration under administration of the selective antagonist in particular. The results also suggest the role of TRPC4 in the regulatory signal pathways in case when impaired contractility have not been treated by oxytocin. A perspective to use liposomes with incorporated agonists of maxi-K channels as the inhibitors of uterine contractions was shown in experiments whith quercetin, in which this compund reduced the frequency of contractions to 30% of control value. We suggest that in mechanisms involving ion channels mentioned above, pharmacological modulators a certain role belongs to calcium sensitivity of the contractile apparatus and to the calcium induced calcium release from sarcoplasmic reticulum. A specific inhibitor of a plasma membrane calcium pump, calix[4]aren C-956, stimulates the increase of Ca2+ concentration in cytosol of smooth muscle cells of both pregnant and non-pregnant rats. Another inhibitor of the plasma membrane calcium pump, calix[4]aren C-1087 (20 µM), similar to the calix[4]aren C-956, increases the Ca2+ concentration in uterine myocytes of non-pregnant rats. However, his effect on the Ca2+ concentration in the uterine myocytes of pregnant rats proved ineffective.   Product Description popup.authors Babich Lidiia H. Veklich Tetiana O. Danylovych Ganna V. Danylovuch Yuriy V. Zholos Oleksandr V. Moroz Olesia F. Nozdrenko Dmytro M. Nuryshchenko Nataliia Ye. Shkrabak Olexandr A. Shlykov Serhii H. popup.nrat_date 2020-04-02 Close