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Information × Registration Number 0220U101074, 0119U104021 , R & D reports Title Тo study of B.canis circulation in pet and stray dogsin Kharkiv region popup.stage_title Head Bolotin Vitaliy I., Zavgorodnii Andrii І., Доктор ветеринарних наук Registration Date 31-01-2020 Organization National Scientific Center Institute of Experimental and Clinical Medicine popup.description2  Wаs determination of epizootic situation of brucellosis of domestic and stray dogs in Eastern Ukraine. In total 190 samples of blood serum from domestic and stray dogs in Donetsk, Luhansk, Dnipropetrovsk, Kherson and Kharkiv regions were examined. According to the results, all samples were negative for Rose-Bengal Test. Using the R-brucellosis antigen, 62 (32.6%) samples were reacted, but further studies on CFT and AGID revealed only 58 (31.6%) and 20 (20.5%) of them, respectively. At the same time, the largest percentage of positively responding dogs for RSAT is observed among stray animals in Donetsk (53.3%), Lugansk (48.4%) and Dnipropetrovsk (34.4%) regions. According to the results of bacteriological studies in the first passage it was not possible to isolate B. canis, however, according to molecular-genetic studies, the genetic material of the pathogen was present in real-time PCR with the Ct value at the level of 35 to 37, which indicates a low concentration of the pathogen in the material . Preliminary results from serological studies indicate the circulation of B. canis among dogs in Ukraine, which can lead to significant economic losses in commercial breeders of dogs and the risk of spreading this infection to people in contact with them. More detailed research is needed to develop strategies for the control of dog brucellosis and outbreaks of this disease in order to prevent infection in dog shelters and nurseries in Ukraine. Product Description popup.authors Dragut Svitlana S. Obukhovska Olga V. Orlov Sergey M. Solodiankin Oleksii S. popup.nrat_date 2020-04-02 Close