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Information × Registration Number 0220U101324, (0119U001677) , R & D reports Title Study of the mechanism of action of new genes in the regulation of riboflavin synthesis in flavinogenic yeast Candida famata popup.stage_title Клонування гену C. famata та Debaryomyces hansenii, гомологічного гену BCRP ссавців, конструювання штамів C. famata з надекспресією цих генів та вивчення здатності до надсинтезу рибофлавіну. Head Sibirny Andriy A., Доктор біологічних наук Registration Date 07-02-2020 Organization Institute of Cell Biology of the National Academy of Sciences of Ukraine popup.description1 popup.description2  A yeast integrative plasmid containing the D. hansenii or C. famata gene encoding a transporter involved in riboflavin excretion under the control of the C. famata TEF1 promoter were constructed. C. famata strains with overexpressed BCRP (breast cancer resistance protein) gene encoding riboflavin excretion system were obtained. The introduction of an additional copy of the BCRP gene D.hansenii or C. famata in the riboflavin overproducers of C. famata, leads to an increase GTP-cyclohydrolase activity and riboflavin production and does not reduce the stability on the sign "RF oversynthesis". It has been shown that riboflavin accumulation in C. famata yeast culture can be increas by enhancing the excretion of this vitamin. Product Description popup.authors Andreieva Yuliia A. Dzanaeva Lubov S. Dmytruk Kostiantyn V. Kurylenko Olena O. Pavliukh Khrystyna V. Petrovska Yana O. Faiura Liubov R. Fedorovych Dariya V. Tsyrulnyk Andrii O. popup.nrat_date 2020-04-02 Close

Information × Registration Number 0220U101324, (0119U001677) , R & D reports Title Study of the mechanism of action of new genes in the regulation of riboflavin synthesis in flavinogenic yeast Candida famata popup.stage_title Клонування гену C. famata та Debaryomyces hansenii, гомологічного гену BCRP ссавців, конструювання штамів C. famata з надекспресією цих генів та вивчення здатності до надсинтезу рибофлавіну. Head Sibirny Andriy A., Доктор біологічних наук Registration Date 07-02-2020 Organization Institute of Cell Biology of the National Academy of Sciences of Ukraine popup.description1 popup.description2  A yeast integrative plasmid containing the D. hansenii or C. famata gene encoding a transporter involved in riboflavin excretion under the control of the C. famata TEF1 promoter were constructed. C. famata strains with overexpressed BCRP (breast cancer resistance protein) gene encoding riboflavin excretion system were obtained. The introduction of an additional copy of the BCRP gene D.hansenii or C. famata in the riboflavin overproducers of C. famata, leads to an increase GTP-cyclohydrolase activity and riboflavin production and does not reduce the stability on the sign "RF oversynthesis". It has been shown that riboflavin accumulation in C. famata yeast culture can be increas by enhancing the excretion of this vitamin. Product Description popup.authors Andreieva Yuliia A. Dzanaeva Lubov S. Dmytruk Kostiantyn V. Kurylenko Olena O. Pavliukh Khrystyna V. Petrovska Yana O. Faiura Liubov R. Fedorovych Dariya V. Tsyrulnyk Andrii O. popup.nrat_date 2020-04-02 Close