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Information × Registration Number 0220U101387, 0118U005173 , R & D reports Title Development and optimization of biosensor monitoring system of neurotransmitters for express diagnostics and control of the effectiveness of treatment of neuropathology. Section 2. Development, optimization and experimental exploitation of biosensor smart systems for monitoring neurotransmitters popup.stage_title Head Soldatkin Oleksandr O., Доктор біологічних наук Registration Date 10-02-2020 Organization Institute of Molecular Biology and Genetics of NAS of Ukraine popup.description2  In the course of the work, a biosensor was developed to determine lactate for neuromonitoring needs, as well as an approbation of the developed glutamate-sensitive biosensor when working with real samples. The optimum conditions for the lactate oxidase immobilization in the production of the lactate-sensitive biosensor were selected. The effect of solution parameters on the functioning of the biosensor (pH, buffer capacity and ionic strength) was investigated. The developed lactate biosensor showed good reproducibility of responses (standard error of determination did not exceed 1.4%) and high operative stability of signals throughout the week. The linear range of lactate determination was from 5 to 1000 μM, with a minimum lactate determination limit of 3 μM, and a sensitivity of 204 nA / mm. A methodological approach for the analysis of glutamate concentrations in samples of rat brain synaptosomes was developed. The presence of a basic signal in the preparation is shown in the absence of exogenous glutamate, which indicates the presence of endogenous glutamate in the samples. Baseline extracellular glutamate level was 7.9 ± 0.6 μM. The rate of glutamate accumulation (1 min) and the glutamate accumulation rate (10 min) were also determined by two biosensor methods and radioisotope analysis. Product Description popup.authors Bakhmachuk Anna O. Dziadevych Sergii V. Kostiuchenko Nadiia I. Mruga Daryna O. Shkotova Liudmyla V. popup.nrat_date 2020-04-02 Close