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Information × Registration Number 0220U101484, 0116U003489 , R & D reports Title The methods of optimization of cryopreservation of tissue twisted tubules of the testes of rats using polymeric nanomaterials and carrier popup.stage_title Head Goltsev Anatoliy M., Доктор медичних наук Registration Date 12-02-2020 Organization Institute of Cryobiology and Cryomedicine, National Academy of Sciences of Ukraine popup.description2  The use of collagen and fibrin gels as constituents of cryoprotectants was found to offset the toxic effects of DMSO, glycerol, and PEO-400 on the spermatogenic epithelial cells of sexually immature rat rats and contribute to a 45 minute increase in the expulsion time of function. Cryopreservation of the convoluted tubules of the testes of pubescent rats under program 1 is most effective when using fibrin gel in combination with 6% glycerol. Application of program freezing (program 2) is most effective when using fibrin gel in combination with 5% DMSO. Indicators of viability, total activity of lactate dehydrogenase, MTT test and histomorphometry of tissue samples of convoluted tubules of testes rats vitrified with use of cryoprotectant medium 1 (DMSO, glycerol, and sucrose compared with For specimens of convoluted tubules of the testes of pubescent rats cryopreserved with uncontrolled slow cooling rate, a water bath at 40°C with pre-incubation of them in liquid nitrogen vapor is the optimal mode of heating. For vitrified samples of convoluted tubules of the testes of pubescent rats, the optimum heating mode is rapid heating by transferring the samples directly to a medium with a temperature of 50 ° C. The results of biochemical, histological and immunohistochemical studies have shown that the addition of carbon nanoparticles (C60) at a concentration of 15 µg/ml or 2% BSA is effective for the rehabilitation of cryopreserved using slow cooling rates of convoluted tubules of the testicles of rats. For vitrified samples of convoluted tubules of the testes of rats, the best preservation of morpho-functional characteristics is determined by using carbon nanoparticles at a concentration of 15 μg / ml or BSA at a concentration of 5%. Product Description popup.authors Volkova Natalia O. Sokil Larysa V. Stepaniuk Liudmyla V. Chernyshenko Liudmyla H. Yukhta Maria S. popup.nrat_date 2020-04-02 Close