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Information × Registration Number 0220U101646, 0115U005023 , R & D reports Title Identification of autophagy regulating genes that mediate drought and salt stress tolerance of crops using tissue-specific transcriptional analysis popup.stage_title Head Korhovyy Vitaliy I., Кандидат біологічних наук Registration Date 17-02-2020 Organization Institute of Food Biotechnology and Genomics of the National Academy of Sciences of Ukraine popup.description2  The main purpose of this phase of the project was to perform bioinformatic analysis of transcriptional profiling. The result of this task with the application of bioinformatics methods is to identify patterns of regulation of the autophagy process under stressful conditions. Comparison of plant expression profiles with different stress-tolerance potentials has made it possible to identify specific sets of tissue-specific resistance to drought and salt stress mediated by autophagy in barley cells. The technique developed should extend the ability to analyze other drought and salt stress cereals genotypes to further search for useful genes, and the data obtained are suitable for further transcriptomic analysis. The purpose of the project phase was to conduct a plausible assessment of the levels of gene expression involved in autophagy in plant cells. As a result, an optimal methodological procedure was developed, including the processes of mRNA isolation, cDNA production, and real-time PCR, which met the goals as much as possible. The identified and selected resistance genes are recommended for use in molecular genetics and breeding as molecular markers. The identified resistance genes have great potential for their active use in plant biotechnology to create new salt- and drought-tolerant varieties of crops, which can greatly improve agricultural efficiency and food security. Product Description popup.authors Yemets Alla I. Karpov Pavlo A. Korhovyy Vitaliy I. Spivak Svitlana I. popup.nrat_date 2020-04-02 Close