1 documents found
Registration Number

0220U101655, 0115U005201 , R & D reports

Title

Obtaining cell lines of agricultural plants with increased resistance to pathogenic and parasitic organisms by the way of inducing of RNA interference process using microbial bioregulators

Head

Spivak Svetlana I., Кандидат біологічних наук

Registration Date

17-02-2020

Organization

Institute of Food Biotechnology and Genomics of the National Academy of Sciences of Ukraine

Description

 The new lines of potato plants (Solanum tuberosum L.) cultivar Vernissage with improved morphogenetic potential and genetically-mediated resistance to the parasitic nematode H. schachtii were obtained under in vitro conditions on nutrient media with bioregulators of microbiological origin used in concentrations: 25, 50, 75 and 100 μl/l. The viability index (%) of experimental potato plants-regenerants grown on the invasive background on nutrient media with bioregulators was found to be increased to 54-79 %, compared with control plants, among which the number of viable plants was 39 %. The degree of damage of the total leaf and stem surface in plants obtained on nutrient media with bioregulators is shown to be reduced to 31–48 %, compared to increase to 67 % degree of damage of control plants-regenerants. The dot-blot hybridization method showed the decrease to 36 – 65 % in the index of the hybrid cytoplasmic mRNA and si/miRNA molecules, isolated from cells of control and experimental potato plants-regenerants, grown under in vitro conditions on the artificial invasive background on nutrient media with bioregulators, compared with the same index of control plants. The experiments conducted under in vitro cell-free system of protein synthesis from wheat seedlings showed an increase in the silencing activity to 31 – 59 % on the template of plant mRNA of si/miRNA isolated from cells of experimental potato plants-regenerants, grown under in vitro conditions on the artificial invasive background on nutrient media with bioregulators, compared with activity of cytoplasmic si/miRNAs isolated from control potato plants-regenerants.

Product Description

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Iutynska Galyna O.
Biliavska Liudmyla O.
Spivak Svitlana I.
Tsygankova Viktoriia А.
Shysha Elena M.

R & D report
Obtaining cell lines of agricultural plants with increased resistance to pathogenic and parasitic organisms by the way of inducing of RNA interference process using microbial bioregulators
Head: Spivak Svetlana I.. Obtaining cell lines of agricultural plants with increased resistance to pathogenic and parasitic organisms by the way of inducing of RNA interference process using microbial bioregulators. Institute of Food Biotechnology and Genomics of the National Academy of Sciences of Ukraine. № 0220U101655
1 documents found

Updated: 2024-07-27


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