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0220U101656,
0115U005025
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Establishment of molecular genetic markers for differentiation of various plant genotypes by studying gene intron polymorphism of cytoskeleton proteins
Pirko Yaroslav V., Кандидат біологічних наук
17-02-2020
Institute of Food Biotechnology and Genomics of the National Academy of Sciences of Ukraine
The exon-intron structure of plant cytoskeletal protein genes (α-, β- tubulin and actin) was analyzed and new universal DNA markers were developed to evaluate the intron length polymorphism of these genes in the A. thaliana, rice (O. sativa), flax -dog (L. anditatissimum), potatoes (S. tuberosum) and tomato (S. lycopersicum). The conditions for amplification of the introns of the α-, β-tubulin and actin genes in A. thaliana, O. sativa, L. and sitatissimum, S. tuberosum and S. lycopersicum using the appropriate species-specific primers were selected. The polymorphism of the intron lengths of α-, β-tubulin and actin genes in higher plant species was analyzed and specific DNA profiles were identified in different plant species and genotypes, which allowed them to be effectively differentiated. The same efficiency of using species-specific DNA markers to detect the polymorphism of the length of the introns of actin genes was shown in comparison with SSR markers by the example of analysis of genetic diversity in tomato varieties. It is established that the developed marker systems make it possible to quickly and reliably differentiate individual genotypes and plant species, and therefore they can be considered as a promising and effective tool for molecular genetic analysis.
Blume Yaroslav B.
Pirko Yaroslav V.
Postovoytova Anastasiya S.
Rabokon Anastasiia M.
2020-04-02
Updated: 2025-12-07
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