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Registration Number

0220U101657, 0115U005200 , R & D reports

Title

The study of role of membrane potassium channels from rice and barley in maintaining mechanisms of osmotic and mineral homeostasis in plants

Head

Isayenkov Stanislav V., Доктор біологічних наук

Registration Date

17-02-2020

Organization

Institute of Food Biotechnology and Genomics of the National Academy of Sciences of Ukraine

Description

 Around 69% of the world's wheat crop harvest is adversely affected by drought and salinisation (about $ 200 million) (FAO, 2014). Further soil salinisation of arable lands is a result of agricultural irrigation and drought and has very negative effects on humanity (loss of 30% of land by 2025 and 50% of land by 2050) (FAO, 2014). One of the promising approaches to increase plant resistance to salinity and water scarcity is enrichment of plant tissues by potassium. Therefore, the introduction or overexpression of genes encoding potassium transport proteins is an important strategy for creating plants with improved resistance to drought, saline and mineral homeostasis. Thus, this project is focused on estimation of the potassium TPK channels roles in plant physiological processes and stress response formation to salinity and drought. The main idea of this project is technology development for creation the plants enriched by potassium. It is expected that these plants will be less demanding to mineral soil composition and more resistant to salinity and drought. As a result of this project, the gene of the two-poure HvTPKc potassium channel from barley was introduced to tobacco plants by agrobacterial transformation. Regenerated plants expressing this transgene were obtained. At this stage, active work was conducted on the collection and selection of seeds from regenerated plants for further molecular-biological and physiological analyses. Developing stress-resistant and potassium-enriched plants can significantly help reduce environmental and economical costs of agriculture. Therefore, the practical significance and economic impact of the implementation of this technology will be extremely important.

Product Description

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Isayenkov Stanislav V.
Kvasko Anna Yu.
Malienko Vadim A.