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Registration Number

0220U101711, 0117U001023 , R & D reports

Title

To study the reparative properties of bioengineered structures on the basis of bone allografts and xenografts that are saturated with stem cells and growth factors

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Head

Korzh Mykola O., Доктор медичних наук

Registration Date

18-02-2020

Organization

State institution "Sytenko Institute of Spine and Joint Pathology National Academy of Medical Sciences of Ukraine"

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 Object of study: osteoregeneration of rabbit humerus defects after plastics by dehydrated bone allografts and xenografts that are saturated with growth factors and stem cells. Purpose: To study in the experiment the course of reparative osteogenesis using the bioengineered structures on the basis of bone allografts and xenografts in combination with autologous serum growth factors and cultured bone marrow stromal cells. Research methods and apparatus: biochemical (electrophotocolorimeter KPK-3, analyzer GBG STAT FAX 1904), enzyme immunoassay (analyzer LisaScan) histological (microscope Olympus BX63), cell cultivation (incubator EC 160, microscope Olympus CKX 31), electron microscopy (EMB-100BР), statistical. Theoretical and practical results: It is established that the highest osteoblastic activity and the most optimal conditions for the bone tissue formation are observed in the case of bone allografts saturated with autologous serum bioactive factors, while the bone xenografts exhibit low osteointegration and are replaced mainly by connective tissue due to the immunoinflammatory reaction and delayed osteoregeneration. The osteoinductive effect of serum bioactive factors on the reconstruction and osteointegration of created bone grafts was more pronounced than the effect of cultured stem cells. Novelty: It was first developed a processing method of bone implantation material on the basis of which the bioengineered structures with osteoinductive properties were created due to of serum bioactive factors or cultured bone marrow stromal cells, and the most effective of these implants for osteoplasty was determined in the animal experiment. Implementation efficiency: The effectiveness of surgical treatment is increased by 15-30%, complications in patients after osteoplastic surgery are reduced by 40%. Field of use: medicine.

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Velyaminova Viktoriya V.
Vyrva Oleksandra O.
Vorontsov Petro M.
Vorontsova Mariia P.
Harbuzniak Iryna M.
Golovina Yanina O.
Hrytsenko Anton V.
Danyshchuk Zinaida M.
Korzh Mykola O.
Leontieva Frida S.
Maltseva Valentyna Ye.
Nikolchenko Olga A.
Orlenko Anton O.
Poshelok Denys M.
Samoilova Kateryna M.
Sereda Olena S.
Khmyzov Sergey O.
Shevtsova Oksana V.
Yakushkin Yevhenii Yu.