Search academic texts

Information × Registration Number 0220U101717, 0120U100380 , R & D reports Title The influence of cryopreservation factors during vitrification on morphofunctional characteristics of the reproductive cells and embryos. popup.stage_title Head Petrushko Maryna P., Доктор біологічних наук Registration Date 19-02-2020 Organization Institute of Cryobiology and Cryomedicine, National Academy of Sciences of Ukraine popup.description2  The object of the study is the viability of human sperm and embryos after cryopreservation by the slow method and by vitrification. The purpose of research is to select the vitrification conditions and determine the quality criteria for sperm and pre-implantation human embryos and compare them with other cryopreservation methods. Research methods – cryobiological, morphometric, light microscopy, fluorescence microscopy, statistical analysis. Results. Two-stage cryopreservation of sperm from pathospermial ejaculates in microcontainers under the protection of 15% glycerol allows to obtain the preservation of single sperm at the level of 90%. Morphological analysis of sperm indicates that sperm with head pathology have a high survival rate after cryopreservation. The index of penetration of ZP by spermatozoa from normozoospermic ejaculates is significantly higher than at oligoastenoteratozoospermia. It is established that the optimal stage for cryopreservation of preimplantation human embryos is the slow method of zygote, and the method of vitrification – blastocyst. The overall survival rate of embryos at the blastocyst stage after vitrification was 94.3 ± 8.8 and (77.5 ± 7.8)% when evaluated immediately after thawing and 3 h respectively. These rates were significantly higher than in embryos, frozen and frozen slowly - 64.4 ± 5.2 and (39.2 ± 4.1)%. Product Description popup.authors Buderatska Natalia O. Butskyi Kyrylo I. Hapon Hanna O. Mikson Kostyantyn B. Pavlovych Olena V. Petrushko Maryna P. Prokopyuk Volodymyr Yu. Puhovkin Anton Yu. Yurchuk Tayisiya O. popup.nrat_date 2020-04-02 Close