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Information × Registration Number 0220U101732, 0120U100056 , R & D reports Title Diversity of endophytic microbial communities of Antarctic vascular plants popup.stage_title Head Zavirukha Ganna A., Кандидат біологічних наук Registration Date 19-02-2020 Organization State Center of Innovation Biotechnologies popup.description2 DNA was extracted with three different protocols from the samples of D. antarctica and C. quitensis. CTAB method was established to be the optimal one for C. quitensis. As to D. antarctica biomass, the DNA can be extracted either by the CTAB method or with spin columns. In both cases, the use of the phenol-chloroform method is impractical. DNA quality and quantity was estimated spectrophotometrically and with gel electrophoresis. The optimal annealing temperatures and PCR conditions were established for 16S rRNA region targeting Actinobacteria, Pseudomonas, Cytophaga-Bacteroidetes, Planctomycetes, and Firmicutes. The obtained amplification protocols are recommended to be used with D. antarctica and C. quitensis plant material. The PCR products were purified, cloned and sequenced for the purpose of phylogenetic analysis. 45 OTUs were obtained, including those phylogenetically related to Actinobacteria, Cytophaga-Bacteroidetes, Firmicutes and Pseudomonas, as well as many uncultured bacteria. PCR was performed using the optimized amplification protocols with the purpose to examine Antarctic vascular plants' microbial communities quantitative composition. Product Description popup.authors Balabukha Liudmyla V. Vasilieva Tetiana A. Davydenko Tetiana I. Diachenko Iryna I. popup.nrat_date 2020-04-02 Close