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Information × Registration Number 0220U101954, 0117U002283 , R & D reports Title Improvement of methods laboratory diagnostic cryptosporidiosis popup.stage_title Head Pokhyl Serhii I., Доктор медичних наук Registration Date 28-02-2020 Organization State Institution "I.Mechnikov Institute of Microbiology and Immunology National Academy of Medical Sciences of Ukraine" popup.description2  The procedures for cryptosporidium oocysts concentrating and purifying for their microscopic detection in the stools were optimized by using the faecal parasitological concentrator “Mini Parasep® SF - EU Facal Parasite Concentrator” (“Apacor Ltd.”, England).For high-differential luminescent-microscopic detection of Cryptosporidium spp. has been developed a safer (phenol-free) and less labor-intensive (almost 2-fold) auromin-rhodamine water method (ARWM) staining their.The technology of production of complete oocyst antigens of Cryptosporidium (POAgCrypto) was mastered and 3 series of their experimental samples were created.According to the results of the study, it was shown that the indirect immune fluorescence reaction reproduced using POAgCrypto is characterized by sufficient discrimination to identify groups of persons with different levels of post-invasive status and can be used to determine the sero-prevalence of cryptosporidiosis.Is developed a more technically accessible and less costly (1.7 times) multiplex polymerase chain reaction (PCR MF) variant for simultaneous detection / identification (with sufficient sensitivity, specificity and reproducibility of the result) in stools of the DNA of both pathogens of cryptosporidiosis and giardiasis. Product Description popup.authors Kostyria Iryna A. Lipovetska Nadija O Pokhyl Serhii I Timchenko Olena M Torianyk Inna I Chyhirynska Nila A popup.nrat_date 2020-04-02 Close