Search academic texts

Information × Registration Number 0220U102194, 0115U000093 , R & D reports Title Development of low-temperature preservation technologies for immobilized cells and bioactive compounds popup.stage_title Head Gulevskyy Oleksandr K., Доктор біологічних наук Registration Date 11-03-2020 Organization Institute of Cryobiology and Cryomedicine, National Academy of Sciences of Ukraine popup.description2  The effect of different modes of destruction of cord blood cells by slow and high freezing / heating rates and high-temperature destruction of blood samples in comparison with the mode of hypotonic lysis on the release of biologically active compounds was studied. It was found that the yield of substances to the fraction of 10 kDa in cryodestruction according to their dry weight is much higher compared to the destruction of cord blood under conditions of hypotension. The chromatographic profiles of the low molecular weight (below 10 kDa) fractions are significantly different, indicating that the composition of the substances in the respective extracts can be varied by the conditions of cell destruction before fractionation, including freezing / heating modes. The destruction of cells by low and high (70oC) temperatures leads to a significant increase in hydrophobic substances in the respective fractions below 10 kDa, namely bound and free testosterone, creatinine and doesn’t affect the amount of hydrophilic substances (calcitonin). It was found that the low molecular weight fraction of cord blood obtained after cryodestruction and hypotonic cell lysis significantly contributed to the increase of ATP in donor red blood cells subjected to hypothermic storage for 14 and 21 days. Product Description popup.authors Akhatova Yulia S. Gorina Olga L. Zharkova Yevheniia Ye. Moiseyeva Natalya M. Nikolchenko Andrii Yu. Chaplai Olena V. Shcheniavskyi Ivan Yo. popup.nrat_date 2020-04-02 Close