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Information × Registration Number 0220U102359, 0118U005178 , R & D reports Title Development, optimization and testing of printed ampereometric epidermal sensing system for the non-invasive detection of L-lactate popup.stage_title Head Korpan Yaroslav I., Кандидат біологічних наук Registration Date 12-03-2020 Organization Institute of Molecular Biology and Genetics of NAS of Ukraine popup.description2 In 2019, research on the creation of an effective biosensor enzyme matrix for the determination of L-lactate in sweat was carried out. To perform this task, a series of laboratory prototypes of biosensor were developed based on immobilized lactate dehydrogenase and NAD+ . The method of immobilization and stabilization of lactate dehydrogenase and NAD + on the surface of a NADN-sensitive electrode by the crosslinking technique in a BSA gel was developed and optimized. Optimized biomatrix and its basic analytical characteristics are studied. It is established that the developed model of the biosensor allows to measure lactate in the concentration range of 1-8 mM with a sensitivity of 0.145 µA / mM and a detection limit of 50 µM. The influence of the interferences on the results of the measurement of lactate with the help of a laboratory model of the biosensor was studied. Biosensor design was optimized with additional membranes. It is shown that an additional polymeric membrane based on m-polyphenylenediamine significantly improves the selectivity of the biosensor. Optimized conditions for the determination of lactate using the developed biosensor (0.3 V, 0.1 M phosphate buffer, pH 7.5, 20 mm NAD +). Product Description popup.authors Biloivan Olga A Korpan Yaroslav I popup.nrat_date 2020-04-01 Close