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Registration Number

0220U103000, 0115U002084 , R & D reports

Title

Genomics and cell biology of plant cytosceleton as tools for it functional and structural study and development of novel biotechnologies

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Head

Blume Yaroslav B., Доктор біологічних наук

Registration Date

13-07-2020

Organization

Institute of Food Biotechnology and Genomics of the National Academy of Sciences of Ukraine

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 The functional role of stress post-translational modifications of tubulin, in particular acetylation, in the implementation of physiological and stress-induced autophagy has been elucidated. A search and identification of protein kinases of plants and animals directly involved in the phosphorylation of α-, β- and γ-tubulins, as well as a search for calcium-dependent protein kinases associated with the regulation of the cytoskeleton of plants and animals, identified appropriate phosphorylation sites on the surface of molecules tubulins. The spatial structure of protein kinases and phosphatases involved in the regulation of the cytoskeleton has been reconstructed. Methods of biological synthesis of CdS quantum dots using the bacterium Escherichia coli, a plant - roots of Linaria maroccana, have been developed. The structural and morphological features of the synthesized CdS nanoparticles were investigated using TEM methods and electrography. A method for the functionalization of CdS quantum dots (P. ostreatus) using dextran polyacrylamide has been developed. Molecular genetic markers have been developed based on the analysis of nucleotide sequences of conserved regions of homologues of genes of cell cytoskeleton proteins (actin and tubulins) in different species of higher plants. The role of the cytoskeleton in intercellular communication and intracellular compartmentation of plant tissues was elucidated. It is shown that the cells of chlorenchyma (C4) in the leaves of bicameral C4 species are characterized by dynamics in their structure and change quite easily in response to changes in environmental conditions.

Product Description

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Yemets Alla I.
Blume Rostislav Ya.
Blume Yaroslav B.
Borova Mariya M.
Buy Denis D.
Horiunova Inna I.
Danylenko Ihor A.
Demchuk Oleh M.
Karpov Pavlo A.
Kravets Olena A.
Krаsylenko Yuliia A.
Kurylenko Svitlana V.
Lytvyn Dmytro Ivanovych
Novozhilov Dmitry О.
Ozheredov Sergey P.
Pirko Yaroslav V.
Pastuchova Nataliia L.
Pydiura Nikolay O.
Plokhovska Svitlana H.
Pushkarova Nadiia O.
Rayevsky Olexey V.
Samofalova Dariya O.
Sakhno Liudmyla O.
Spivak Svitlana I.

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2020-10-01