Search academic texts

Registration Number

0220U103133, 0115U006668 , R & D reports

Title

Scientific basis of application of methods of detection and determination of GMOs in agricultural plants and food products

popup.stage_title

Head

Yemets Alla I, Доктор біологічних наук

Registration Date

17-08-2020

Organization

Institute of Food Biotechnology and Genomics of the National Academy of Sciences of Ukraine

popup.description2

 The paper provides an overview of the most commonly used and accepted in most countries of the world methods of sampling and DNA extraction both from seeds and from processed products. The system for the development and validation of detection methods is considered, which is an important prerequisite for ensuring high quality and reliable data in GMO testing among reference laboratories in the EU Member States. Methods based on DNA analysis - the most widespread and universal due to the stability and relatively easy availability of this molecule - are considered. Minimization of amplification processes, increasing the sensitivity of detection methods allow analyzing huge arrays of DNA isolated from seeds, food, feed, etc., for a short time. Most of the methods have been developed on the basis of PCR in various variations. The most promising is the development of the direction of biochips - in terms of versatility, speed, accuracy and convenience in carrying out analyzes. Among the most widespread and promising for development in the field of detection are such methods as: high performance liquid chromatography; spectroscopy in the near infrared (Near infrared (NIR) spectroscopy) detection based on biosensors - surface plasmon resonance; surface-enhanced Raman spectroscopy. For the detection of GMOs, protein analysis methods are used, which are based on enzyme-linked immunosorbent assay (ELISA). The main advantage of these methods is the simplicity of the analysis - from sample preparation to obtaining the final result, the speed of obtaining reliable results. A very affordable immunochromatographic test (ICT) has been developed - compact and easy to use. ICT is a semi-quantitative method for screening and measuring new distant proteins presented through hereditary plant change. Test strips are the simplest method for evaluating multiple Bt-transformed crops for insecticide resistance.

Product Description

popup.authors

Yemets Alla I.
Korhovyy Vitaliy I.
Levchuk Oleg M.
Patsyuk Fedir N.
Yatsyshyn Valentyna Yu

popup.nrat_date

2020-10-01