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Information × Registration Number 0220U103183, 0114U000907 , R & D reports Title Investigation of the role of ion channels, receptors and synaptic proteins in the regulation of calcium-dependent synaptic transmission in health and disease popup.stage_title Head Lukianets Olena O, Registration Date 25-08-2020 Organization Bogomolets Institute of Physiology popup.description2 Investigate of the role of TRP-type calcium ion channels (TRPV1 and TRPA1 channels) in the regulation of normal synaptic transmission and pathology. The level of expression of genes encoding these channels was investigated by PCR. The expression of TRPV1 channel genes in these cells was shown and it was found that the expression level of TRPV1 channels in chromaffin cells is much lower than in sensory neurons, and for activation of TRPV1 channels in chromaffin cells, much higher agonist concentrations are required for their activation. Studies of the activity of TRPA1 and TRPV1 channels in sensory neurons have shown that repeated application of a TRPV1 channel agonist led to their desensitization. Subsequent activation of TRPA1 channels led to the restoration of the sensitivity of TRPV1 channels (reset). Thus, the presence of regulation of TRPV1 channel activity by TRPA1 channels was established, which indicates a close interaction of TRPV1 and TRPA1 channels in sensory neurons. Determination of the coexpression of TRPV1 and TRPA1 channels in single cells by single cell PCR showed a clear correlation between the presence of channel activity and their expression. Thus, our studies have shown that TRP-type calcium channels can interact by influencing each other's function in the sensory information transmission system.  Product Description popup.authors Burlak Oleksii P. Ganzha Vita V. Lukyanets Olena O. popup.nrat_date 2020-10-01 Close