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Information × Registration Number 0220U104256, 0120U104574 , R & D reports Title Combined test systems for diagnosis and analysis of innate immunity gene expression upon dangerous viral infections popup.stage_title Head Tukalo Mykhailo A., Доктор біологічних наук Registration Date 15-12-2020 Organization Institute of Molecular Biology and Genetics of NAS of Ukraine popup.description2  Purpose: Development of combined test systems as a means of effective diagnosis of particularly dangerous viral infections (ONVI) and coinfections, which also include the ability to analyze the expression of innate immunity genes in these diseases. As a result of the first stage of the work, the analysis of the nucleotide sequence of the genomes of a number of strains of SARS-CoV-2 virus, influenza and other particularly dangerous viral infections (HDVI). Bioinformative analysis of genomes and design of primers for the creation of test systems based on isothermal and PCR analyzes were performed. Literature data and own previous studies were processed to determine the list of innate immunity genes. Specific oligonucleotides were selected for PCR analysis of pathogenic viruses and expression of human immune system genes for research, development and testing of test systems. Biological fluids of healthy and infectious patients were collected and RNA and cDNA collections were created. Techniques for obtaining RNA from scrapings for direct detection of gene expression for PCR analysis and by using Bst3.0 isothermal polymerase were developed. The recombinant Bst DNA polymerase enzyme was cloned, expressed in bacterial cells and developed to create an express test system based on isothermal PCR. Product Description popup.authors Gerashchenko Ganna V. Gudzera Olga I Kashuba Volodymyr I Marchyshak Tetiana V. Melnichuk Nataliia S. Nikolaiev Roman O. Nuzhny Olexander O. Tkachuk Zenoviy Yu. Tukalo Mykhailo A. popup.nrat_date 2020-12-15 Close