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Information × Registration Number 0220U104346, 0119U102351 , R & D reports Title Mechanisms of epileptiform activity and properties of hippocampal synaptic plasticity due to dysfunction of the blood-brain barrier popup.stage_title Head Savotchenko Alina V., Registration Date 16-12-2020 Organization Bogomolets Institute of Physiology of the National Academy of Sciences of Ukraine popup.description2  One of the consequences of GEB violation is a change in the ionic composition of the intracerebral environment: the intercellular fluid of the brain acquires the ionic composition of blood plasma in the affected areas. Such shifts in the ionic composition can lead to changes in the impulse activity of neurons and the efficiency of synaptic transmission and, as a consequence, increase the excitability of neural networks. During the project we used model conditions that mimic the effects of GEB disruption in hippocampal neuron culture, which revealed the effect of plasma ionic medium on the parameters of impulse activity of hippocampal neurons and their ability to epileptiform activity, as well as to determine the role of serum thrombin protein in activity of hippocampal neurons. The effect of pharmacological blockade of protease-activated receptors 1 (PAR1) on behavioral disorders of the depressive type after status epilepticus (EC) was also investigated. Behavioral tests were performed during the latent stage of formation of temporal lobe epilepsy, two weeks after EC induced using a lithium-pilocarpine model. PAR1 blocker injections were given 10 days after EC. The obtained results indicate a partial normalization of depressive behavior in the test of "forced swimming". We also observed the recovery of the reactions of experimental rats after the suppression of surfactant function in the "sucrose" test. Inhibition of surfactant led to increased climbing time in the "forced swimming" test. At the same time, the swimming time in the "forced swimming" test decreased as a result blocking of surfactants compared with the control group, and floating time increased. Product Description popup.authors Semenikhina Margaryta O. Shypshyna Mariia S popup.nrat_date 2020-12-16 Close