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Information × Registration Number 0220U104402, 0120U104112 , R & D reports Title SARS-CoV-2 vaccine development based on the yeast expression of S protein fragments and studying immune response in the laboratory animals popup.stage_title Head Sibirny Andriy A., Доктор біологічних наук Registration Date 16-12-2020 Organization Institute of Cell Biology popup.description2 Cassettes for expression of fragments of S1 and S2 subunits of SARS-CoV-2 spike glycoprotein under the control of AOX1 promoter in "humanized" strains of yeast K. phaffii and O. polymorpha were obtained. The expressed fragments will additionally have a fibrin protein trimerization domain, an immunological adjuvant (hIgG1-Fc2), a TEV protease action site, and a 6-His sequence required for efficient protein purification. The fragments will be secreted from the yeast cells into the culture medium due to the presence of a secretion signal - α-factor. Cassettes for expression of full-length S protein, M- and E-proteins of SARS-CoV2 and small S protein (dS, scaffolding protein) of duck hepatitis virus were also obtained for further assembly of SARS-CoV2 virus-like particles. Product Description popup.authors Vasylyshyn Roksolana V. Dmytruk Kostyantyn V. Zazulya Anastasiya Z. Semkiv Marta V. Fayura Lyubov R. Fedorovych Dariya V. popup.nrat_date 2020-12-16 Close