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Information × Registration Number 0220U104426, 0120U104115 , R & D reports Title Development of new nanozymes as catalytic elements for enzymatic kits and chemo/biosensors popup.stage_title Head Gonchar Mychailo V., Доктор біологічних наук Registration Date 17-12-2020 Organization Institute of Cell Biology popup.description2  Carbon-based nano/micromaterials (derivatives of nanotubes, nanowalls, carbonized cellulose) were synthesized and characterized using electron microscopy and positron annihilation spectroscopy. Synthesis of new nanoparticles (NPs) of precious, transition and rare metals, as well as their hybrid forms by chemical reduction of the corresponding salts and by "green" synthesis was done. Scanning electron microscopy and X-ray spectral microanalysis proved their nanoscale state and characterized their chemical composition and morphology. The presence of characteristic absorption maxima in the UV-visible spectra confirms the formation of the corresponding NPs. The analysis of synthesized NPs for pseudoenzymatic activity (peroxidase, laccase, catalase and reductase) was performed. It was shown that among the obtained 25 NPs of different chemical composition, 10 have the highest peroxidase activity, 4 - laccase, one type - catalase and reductase activity. Various nanomaterials based on ferrocyanide salts of iron and other transition metals (analogues of Prussian blue) were synthesized by methods of co-precipitation of the corresponding salts and by "green" synthesis. Among the 12 chemically synthesized hexacyanoferrates, there were found nanozymes with high pseudoperoxidase activity (1.7-3.3 Units/mg) and pseudolaccase activity. Screening and structural-morphological characteristics of magnetic nanoparticles based on iron oxide were performed. It is shown that the modification of magnetic nanoparticles by nanozymes and hemin resulted in an increase (2-1.5-fold) in peroxidase activity in comparison with unmodified materials. The most promising materials were selected for further research. It is shown that carbon materials (carbonized cellulose, carbon nanotubes) have a low peroxidase activity, which after modification with hemin and nanoparticles of precious metals increases significantly (2-15-fold) compared to unmodified materials. Product Description popup.authors Demkiv Olha M. Stasiuk Nataliia Ye. popup.nrat_date 2020-12-17 Close