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Information × Registration Number 0220U104438, 0120U102948 , R & D reports Title Creation of highly effective strains of rhizobia using genetic transformation for the development of agricultural biotechnologies and increasing legume productivity popup.stage_title Head Kots Sergij Ya., Доктор біологічних наук Registration Date 18-12-2020 Organization Insninute of plant physiology and genetics popup.description2  As a result of the conducted researches it is shown that on cultural properties, and also resistance to marker antibiotics (streptomycin, kanamycin) strains-recipients of nodule bacteria of a clover of R. leguminosarum bv. trifolii 348a, BN7 and BN9, as well as lupine Bradyrhizobium sp. (Lupinus) 168 and 359a can be used for Tn5 mutagenesis. At the same time, strains of R.leguminosarum bv. trifolii BK103, BK210 and Bradyrhizobium sp. (Lupinus) 400, 371a, 367a, 176, 373a are not suitable for Tn5 mutagenesis due to non-compliance with the requirements for resistance to marker antibiotics. As a result of insertional Tn5 mutagenesis of clover rhizobia BH7, BN9, 348a and lupine 168 and 359a and verification of the obtained transformed cells on the basis of stability to marker antibiotics, 220 mutant clones of clover nodule bacteria and 60 Tn5 mutants were selected. The frequency of occurrence of Kmr cells depended on the genotype of nodule bacteria involved in Tn5 mutagenesis and was for strains BN7, BN9, 348a, 168, 359a, respectively, 10(-7), 10(-7), 10(-6), 10(-7), 10(-9). Product Description popup.authors Vorobey Nadiya A. Kukol Kateryna P. Melnykova Nataliya M. Mykhalkiv Lyudmyla M. popup.nrat_date 2020-12-18 Close