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Information × Registration Number 0220U104512, 0120U103055 , R & D reports Title Construction of ethanol producers from glycerol based on methylotrophic yeast Komagataella phaffii and Ogataea polymorpha popup.stage_title Head Dmytruk Kostiantyn V., Доктор біологічних наук Registration Date 19-12-2020 Organization Institute of Cell Biology popup.description2 Recombinant strains of K. phaffii with overexpression of heterologous genes ADH1 (encoding alcohol dehydrogenase) or PDC1 (encoding pyruvate decarboxylase) from Saccharomyces cerevisiae under the control of a strong constitutive promoter of the GAP1 gene were obtained. The obtained strains demonstrated increased activities of the corresponding enzymes in comparison with the wild-type strain. During fermentation in a medium containing chemically pure or crude glycerol (which is a waste of biodiesel production), the obtained strains produced 1.5-2.5 times more ethanol compared to the wild-type strain. During the fermentation of crude glycerol, all analyzed strains of K. phaffii, including the wild-type strain, produced more ethanol than during the fermentation of chemically pure glycerol, so K. phaffii is a good platform for creating an effective ethanol producer from crude glycerol. Product Description popup.authors Vasylyshyn Roksolana V. Semkiv Marta V. Sibirny Andriy A. popup.nrat_date 2020-12-19 Close