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Information × Registration Number 0220U104542, 0116U001027 , R & D reports Title Role of endoplasmic reticulum stress in functional reprogramming of genome and identification of target genes for suppressing of glioma growth. popup.stage_title Head Minchenko Oleksandr H., Registration Date 22-12-2020 Organization Palladin Institute of Biochemistry of the National Academy of Sciences of Ukraine popup.description2  The aim of this study was identification of changes in the expression of genes, which control cell proliferation and surviving, and studying the effect of natural and dominant-negative PFKFB4 and ERN1 as well as effect of NAMPT and HSPB8 mRNA silencing on the expression of these genes in glioma cells to elucidate their possible contribution in glioma growth and genome reprogramming to identify genes that can inhibit the growth of malignancies. It was shown that in glioma cells with inhibited IRE1 activity the level of pro-proliferative genes is decreased, but anti-proliferative – decreased and that these changes are mediated as endoribonuclease IRE1 (CDC12, ECM1 та MPC1) and protein kinase of this signaling protein (DNMT1, IDE, PITRM1 та DNAJB9), аbut through different signaling pathways, а as well as by their interaction (IGFBP3, CTGF, IFI30 і TGM2). This indicates the versatility of regulatory mechanisms of gene expressions on transcriptional and post-transcriptional levels. The transfection of glioma cell both kinase dominant-negative PFKFB4 and alternative splice variant of PFKFB4 without kinase activity down-regulates glioma cell proliferation and expression level of most pro-proliferative genes. Product Description popup.authors Efimova Yuliya V. Viletska Yuliia M. Halkin Oleh V. Gnatyk Oksana S. Danilovskyiy Sergiy V. Kryvdiuk Iryna V. Luzina Olha Ya. Minchenko Dmytro O. Ratushna Oksana O. Rudnitska Olha V. Slusar Miroslava Yu. Khita Olena O. popup.nrat_date 2020-12-22 Close