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Information × Registration Number 0220U104544, 0119U001866 , R & D reports Title Natural alkaloids from traditional Chinese medicine (TCM) for treatment of cancer and reverse multidrug resistance. popup.stage_title Head Drobot Liudmyla B., Доктор біологічних наук Registration Date 22-12-2020 Organization Palladin Institute of Biochemistry of the National Academy of Sciences of Ukraine popup.description2  The object of the study: molecular mechanisms that provide the antitumor effect of natural alkaloids homoharringtonine, licorine, and berberine. Aim of the study: to investigate the effects of natural alkaloids homoharringtonine, licorine, and berberine on the proliferation, migration and invasion of tumor cells of different tissue origins, as well as the molecular mechanisms of the realization of their antitumor effects. Research methods: proliferative potential was assessed using MTT test and colony formation test, migration - using in vitro scratch test, invasion - using a modified Boyden chamber. Changes in gene expression levels were investigated by quantitative PCR and Western blot analysis. It was found that the use of homoharringtonine led to decreased levels of proliferative activity of MDA-MB-231breast cancer cells , increased expression of caspases-3, -8 and -9, N-cadherin, VEGFR2, GSK3β, β-catenin, WNT signaling inhibitors DKK1, 2 , 4. Licorine was found to inhibit proliferation and migration of breast cancer cells lines MDA-MB-231 and MCF-7, and to modulate the expression of E- and N-cadherins. Berberine reduced the proliferative potential, motility, and invasiveness of mouse LLC lung cancer cells, along with the decreased expression of adaptor protein Ruk/CIN85 and the cancer stem cells marker CD44. Product Description popup.authors Horak Iryna Iryna R. Skarerna Tetyana D. popup.nrat_date 2020-12-22 Close