Search academic texts

Information × Registration Number 0220U104724, 0115U003743 , R & D reports Title Plant genome variation in extreme environmental conditions popup.stage_title Head Kunakh Viktor A., Registration Date 26-12-2020 Organization Institute of Molecular Biology and Genetics of NAS of Ukraine popup.description2 To study structural and functional variability of plant genome in extreme environments in nature and under the experimental stress conditions, wild growing populations, plants cultured in vitro and tissue culture of Antarctic hair grass (Deschampsia antarctica) were investigated. Molecular-genetic, cytological, biochemical, morphometric (biometric), and ecological-population methods, as well as mathematical analysis were used. The peculiarities of the abiotic environment, the composition of plant cenoses and the natural environment of populations of Antarctic higher plants were studied. The relatively low genetic diversity of the species in Maritime Antarctic, the differentiation of populations from longitudinally distant regions and the tendency to reduced genetic diversity in the north to south direction. A diploid set of chromosomes 2n=26 was determined for D. antarctica plants from Marine Antarctica, and new chromosomal forms of the species were found for the first time. A number of genes encoding stress-induced transcription factors from the DREB family were identified in silico and characterized. The clonal progeny of long-term cultured plants was shown to maintain genetic characteristics that indicates the utility of using the micropropagation technique developed by us for D. antarctica multiplication. The increased tolerance of plants to cadmium compared to other species of vascular plants as well as quantitative changes of pigments under the UV irradiation were found. The amount and profile of phenolic compounds and flavonoids were shown to be generally comparable in cultured and wild plants that gives reason to consider them as a potential source of valuable BAC. Extracts of in vitro plants were found to inhibit the proliferation of pathogenic cells, as well as RNA synthesis by T7 RNA polymerase in the model system. A collection of plants from various parts of Maritime Antarctic was established as well as a bank of D. antarctica DNA. Product Description popup.authors Andrieiev Ihor O. Bublyk Olena M. Konvalyuk Iryna I. Melnyk Vitaliy M. Myriuta Ganna Yu. Mozhylevska Liudmyla P. Navrotska Daria O Parnikoza Ivan Yu. Poronnik Oksana O. Twardovska Maryana O. popup.nrat_date 2020-12-26 Close