Search academic texts

Information × Registration Number 0221U000022, 0120U103320 , R & D reports Title Identification of the components of signalling pathways as markers for the prediction of the invasiveness of breast cancer tumours popup.stage_title Head Rynditch Alla V., Доктор біологічних наук Registration Date 02-01-2021 Organization Institute of Molecular Biology and Genetics of NAS of Ukraine popup.description2 During the current research, we revealed the interaction between SH3 domains of proteins TKS4 and TKS5 with RNA-binding proteins SAM68, LARP6, and WBP11. We also produced plasmids encoding fluorescently labeled deletion mutants of SAM68 that will be used during further work to confirm the identified interaction and to analyze the role of different regions of SAM68 in the formation of specific granules in the cells. For subsequent studies, we created a 293 cell line with the overexpression of membrane-deforming amphiphysin family proteins fused to the omni-tag. For replenishment of the collection of human breast tumor samples, we additionally collected 20 samples of breast tumors. We analyzed the link between the expression of S6K isoforms and indicators of tumor aggressiveness – invasiveness and metastasis. The data obtained indicate that the differential expression of S6K1 isoforms affects the migration of cells inducing morphological changes toward the features specific for the mesenchymal cells. Next, the differential expression of S6K1 isoforms affected the expression of a number of molecules that regulate both intercellular interactions and adhesive properties of the cell. These processes are the steps of the invasion process of malignant tumors and metastasis formation at the body level.  Product Description popup.authors Garifulin Oleg M Gryaznova Tetyana A. Dudchenko Taras M. Kropyvko Sergii V. Krupska Iryna V Pankivskyi Serhii V. Savinska Liliya O Filonenko Valeriy V. Iungin Olga S. popup.nrat_date 2021-01-02 Close