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Information × Registration Number 0221U100279, 0119U100697 , R & D reports Title Study of the role of transcriptional and translational changes of the ATM / CHEK2 / P53 / PUMA molecular pathway in the formation carcinogenic effects in the immune system of Chernobyl clean-up workers in the remote period after exposure. popup.stage_title Head Bazyka Dimitry A., Доктор медичних наук Registration Date 04-01-2021 Organization State Institution "National Research Centre For Radiation Medicine of National Academy of Medical Sciences of Ukraine" popup.description2  The object of study: peripheral blood lymphocytes of 100 Chornobyl clean-up workers (main group), 30 non-exposed persons without oncological pathology (negative control group), 50 persons with oncological pathology (positive control group). Objective: to investigate the relative level of gene expression of the ATM/CHEK2/P53/PUMA cascade and associated genes-candidates in the peripheral blood leukocytes of Chornobyl clean-up workers depending on age, doses of exposure, quantitative genomic instability parameters and the presence of oncological pathology. Methods of research: immunological, molecular genetic, cultural, statistical. Results. Manifestations of genome instability were found in Chornobyl clean-up workers and persons with oncological pathology by the relative telomere length, the distribution of lymphocytes over the phases of the cell cycle, the expression of histone gamma-H2AX and the Cyclin D1 protein. Relationships between the quantitative radiation-induced genomic instability parameters with the expression of genes associated with the ATM/CHEK2/P53/PUMA cascade, which are involved in the regulation of inflammatory processes (TNF, IFNG), telomere length (TERF1, TERF2, TERT), apoptosis ( BCL2, HNRNPA1), antioxidant protection (GSTT1) and oncological transformation (TP53, EGFR, VEGF) in Chornobyl clean-up workers. The markers of genome instability in the remote period after irradiation and early diagnosis of oncological transformation were determined. Product Description popup.authors Ilienko Iryna M Belayev Oleg A Vorobiov Mykola O Golyarnik Nataliya A Zvarych Liliaa M Lyaskivska Olena V Maznichenko Oksana L popup.nrat_date 2021-01-04 Close