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Information × Registration Number 0221U100281, 0120U100749 , R & D reports Title To study the significance of alternative splicing of the SorL1 gene for impaired expression of lipoprotein lipase in patients with chronic lymphocytic leukemia affected due to Chornobyl NPP accident popup.stage_title Head Diahil Iryna S., Chumak Anatolii A., Доктор медичних наук Registration Date 04-01-2021 Organization State Institution "National Research Centre For Radiation Medicine of National Academy of Medical Sciences of Ukraine" popup.description2  The object of study: 130 chronic lymphocytic leukemia (CLL) patients, including 70 ionizing radiation (IR) exposed patients due to the Chornobyl NPP accident (the main group) and 60 IR non-exposed CLL patients (the group of comparison). The aim of work: to establish the value of the lipoprotein lipase gene (LPL) expression for the prognosis of the disease in CLL patients suffered due to the Chornobyl NPP accident. Methods: clinical, polymerase chain reaction, direct sequencing of reaction products, statistical. Equipment: thermal cycler Bio-Rad C1000 (N10450137), dispensers. The main results. The expression of the LPL gene with a high degree of probability allows to correctly assess the mutational status of IGHV genes in 89.2% of CLL patients. Differences in the levels of LPL gene expression depending on the radiation anamnesis of patients were not detected. In patients with unmutated IGHV genes there were revealed: correlation between the levels of LPL and c-MYC gene expression (r = 0.351; p = 0.013), which suggested of higher proliferative activity of leukemic B cells; association between LPL expression level and rs1642785 and rs17878362 polymorphisms of the TP53 gene; significantly higher level of LPL expression in cases with stereotyped B-cell receptors compared with non-stereotyped cases (p = 0.03). Determination of LPL gene expression can be used as a marker to predict the time-to-treatment period, progression-free and overall survival of CLL patients. An increased risk of secondary tumors development in CLL patients with relative LPL expression level more than 300 was found. Field of application: radiobiology, hematology. Publications - 3 papers, patent application, information technology Product Description popup.authors Abramenko Iruna V Bilous Nadia I Martina Zoya V. popup.nrat_date 2021-01-04 Close