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Information × Registration Number 0221U100375, 0116U002078 , R & D reports Title To investigate the biological and antigenic properties of the viruses under the influence of metal nanoparticles popup.stage_title Head Derevianko Stanislav V, Кандидат біологічних наук Registration Date 05-01-2021 Organization Institute of Agricultural Microbiology and Agro-industrial Manufacture of National Academy of Agrarian Sciences popup.description2  The purpose of the work is to study the reproduction of viruses and their antigenic and biological properties under the action of nanoparticles of metals and nonmetals. The threshold limit value (TLV) for bentonite nanoparticles (NPs) is 500 μg/cm3, for Al2O3 and CeO2 – 100 μg/cm3, Ni - 50 μg/cm3, V – 25 μg/cm3, Al – 20 μg/cm3. The TLV for Ti NPs was 12.5 μg/cm3, I + S, Zn, Co - 5 μg/cm3, Se + I – 0.5 μg/cm3. According to the prophylactic and treatment schemes, bentonite, Al2O3, CeO2 and Ce nanoparticles showed the most pronounced antiviral activity against the strain of PTV-1 virus Dniprovskyi 34 among the studied nanoparticles, reducing the virus titer by 1,50-2,00 lg TCD50/cm3. According to the virucidal scheme nanoparticles of Ni, Ti and the composition of I and S nanoparticles showed high virucidal activity against the strain of PTV-1 Dniprovskyi 34, significantly reducing its infectious titer in transplantable culture of porcine embryonic kidney cells (PEKC) by 2,46; 4,46 and 5,00 lg TCD50/cm3, respectively, with TI is 4, 8, and 8, respectively. The biological and physicochemical properties of Teschovirus A Dniprovskyi 34 strain under the influence of nanoparticles were studied in experiments on culture cells PEKC. Based on the results of research, a database on the effects of nanoparticles on the biological and physicochemical properties of Teschovirus A has been developed. The morphological features of nanoparticle preparations have been studied. Under the influence of Ce, Ti, Ni, V, Se and I nanoparticles, spherical virions of the icosahedral symmetry type with a size of 14–22 nm were found, which is much smaller than the sizes of native virions. In addition, virions of round irregular shape with dimensions of 26–28 nm, which by the nature of contrast can be destroyed virions or structural elements of virion capsids have been found. Under the influence of nanoparticles, viruses retain their antigenic properties. Product Description popup.authors Vasylchenko Anatolii V Volkova Izabela V Dmytruk Oksana O Reshotko Leonid M Strekalov Volodymyr M Sheremet Liudmyla I popup.nrat_date 2021-01-05 Close