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Information × Registration Number 0221U100491, 0120U102860 , R & D reports Title Enzymatic pool of macromycetes in bioconversion of lignocellulosic residues popup.stage_title Head Radchenko Volodymyr G., Доктор біологічних наук Registration Date 05-01-2021 Organization Institute for evolutionary ecology of the National Academy Sciences of Ukraine" Ukraine popup.description2  We examined a basidiomycetes ability to decompose lignin using 20 cultures of 15 species, i.e. A-7 - Auricularia auricula Underw., Ab-1 - Agaricus bisporus (JELange) Imbach, Cs-1 - Coriolus sinuosus Bondartsev & Singer, Fvv - Flammulina velutipes (Curtis) Singer, Gl - Gl - Gl - Gan (Curtis) P. Karst., Il-11, Il-15, Il-20 - Irpex lacteus (Fr.) Fr., Ls - Laetiporus sulphureus (Bull.) Murrill, Me - Morchella esculenta (L.) Pers.: Fr., Pe-1 - Pleurotus eryngii (DC.) Quél., Po-1, Po-8, Po-9 - Pleurotus ostreatus (Jacq.) P. Kumm, Rs-1 - Ramaria stricta Quél., Sc- 51, Sc-51-5 - Schizophyllum commune Fr., Sh - Stereum hirsutum (Willd.) Pers., Tv-13 - Trametes versicolor (L.) Lloyd, Tb - Trichaptum biforme (Fr.) Ryvarden). We showed that basidium cultures Il-11, Il-15 and A-7 are the most effective in lignin molecules degradation, besides A-7 has a complete set of oxidizing enzymes required for lignin degradation. The same species cultures show the different ability to lignin transformation indicating their genetic heterogeneity and lack of correlation with the fungi species feature. Decreasing of the medium’s pH increases the expression and activity of enzyme systems involved in the transformation of lignin. The Il-11 culture is capable to express four laccase’s isoforms, and the Il-15 and A-7 cultures are capable to express two isoforms. The Il-11 culture actively decomposes the standard lignin into several large fractions with different physicochemical properties, and the Il-15 and A-7 cultures decompose the high molecular weight fraction of lignin to form a significant number of fractions. Product Description popup.authors Boyko Serрhii M. Netsvetov Maksym V Prokopuk Yuliia S. popup.nrat_date 2021-01-05 Close