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Information × Registration Number 0221U100561, 0120U104770 , R & D reports Title Development of fluorescent diagnostic materials for hybridization assays and studies by polymerase chain reaction popup.stage_title Head Tatarets Anatolii L., Registration Date 07-01-2021 Organization State Scientific Institution Scientific and Technological Complex" Institute of Single Crystals "of the National Academy of Sciences of Ukraine popup.description2  The aim of the work was to create a national fluorescent materials for hybridization analysis, PCR diagnosis of genetic and infectious diseases, in particular COVID-19 virus, determination of the presence of genetically modified organisms (GMOs) and other nucleic acid studies. The purpose of the first stage is to select the most promising prototypes of intercalator dyes for further functionalization and improvement of their properties and to obtain reactive monomeric intercalator dyes that will be suitable for the synthesis of dimeric dyes. During the project implementation, we selected prototypes of fluorescent dyes-intercalators sensitive to the presence of double-stranded DNA (dsDNA) based on the literature and patent data, as well as the results of our many years of experience and testing of dyes from our own library of compounds. Data on the spectral and photophysical properties of fluorescent compounds, potentially suitable as fluorescent dyes-intercalators, were obtained, the ways of their further functionalization in order to improve their properties were proposed. The synthesis of reactive monomer dyes-intercalators was carried out, in particular, derivatives of acridine and unsymmetrical monomethine dyes for further synthesis of dimeric dyes-intercalators were obtained, their structures were confirmed , spectral and photophysical properties in the free state and upon dsDNA binding were investigated. Product Description popup.authors popup.nrat_date 2021-03-17 Close