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Registration Number

0221U100586, 0118U003663 , R & D reports

Title

Study of molecular biological and phenotypic manifestation of transmitted genes performance and their inheritance in biotechnological plants

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Head

Morgun Bogdan V., Кандидат біологічних наук

Registration Date

07-01-2021

Organization

Institute of Cell Biology and Genetic Engineering

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 The object of research was genes nptII, gfp, uidA transferred to wheat and corn plants, rho0 mutants of yeast Saccharomyces cerevisiae and S. paradoxus, hybrids of the Brassica family. The aim of the work was to study the functioning of stress resistance genes, the possibility of transferring foreign genes of different origins to economically valuable plants, to clarify the features of their expression and inheritance, physiological-biochemical and molecular-biological manifestation. Self-pollination was performed and wheat seeds were obtained to study the inheritance of the nptII gene in the T3 generation. In Agrobacterium-mediated transformation in vitro, the transformation efficiency of Yatran 60 cultivar was significantly higher when using strain GV3101 (transformation frequency 3.41±0.95% and 1.28±0.59% for strains GV3101 and C58, respectively). In parent plants of hybrids, rapeseed cultivars Westar and Pactol, Lesquerella fendleri and Orychophragmus violaceus, when grown under stress conditions showed a decrease in the amount of soluble protein, a significant increase in raw weight gain in rapeseed and its reduction for L. fendleri and O. violaceus. In transgenic maize gfp gene, fluorescence peaks characteristic of the fluorescence of mutant GFP S65T were recorded, indicating a high level of green fluorescent protein expression in maize plant extracts. The collection of petit mutants of the yeasts S. cerevisiae and S. paradoxus included petites of 15 laboratory, natural and commercial wine strains. In petit mutants of S. cerevisiae and S. paradoxus, invasion into the nutrient medium was suppressed, while the ability to form aggregate invasive growth was preserved.

Product Description

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Bannykova Mariia O
Nitovska Iryna O.
Pronina Olha V.
Rymar Yuliia Yu

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2021-03-17