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Information × Registration Number 0221U100719, 0119U100217 , R & D reports Title To investigate the formation of effective symbiotic systems of soybeans and evaluate changes in populations of nodule bacteria under introduction of Bradyrhizobium japonicum strains of different genetic groups popup.stage_title Head Krutylo Dmytro V., Кандидат біологічних наук Registration Date 08-01-2021 Organization Institute of Agricultural Microbiology and Agro-industrial Manufacture of National Academy of Agrarian Sciences popup.description2  Object of research - nodule bacteria, binary composition of strains, soybean. The work purpose - to investigate the relationship of soybean varieties with B. japonicum strains, which are characterized slow and intensive growth and belong to different genetic groups; to assess changes in populations of nodule bacteria under introduction of B. japonicum 46 + B. japonicum KB11 strains. Methods of researches: microbiological, physiological and biochemical, serological, vegetative and field experiments, statistical. It was found that strains of soybean rhizobia with different growth rates differ in the level of phytohormones synthesis: intensive-growing strain B. japonicum KC19 synthesised significantly more auxins (44.7 µg/g ADB) and cytokinins (17.0 µg/g ADB) compared to the slowly-growing strain B. japonicum KC23 (1.6 and 1.4 µg/g ADB, respectively), while the slow-growing strain was significantly superior by gibberellin synthesis (205.5 µg/g to 85.0 µg/g ADB). During the cultivation of soybeans in monoculture, a gradual transformation of the poly-strain population of rhizobia into mono-strain population was noted. In the fourth year, only the intensive-growing B. japonicum strain KB11 (genetic group USDA 123) was able to colonize soybean roots, completely displacing the slow-growing strains B. japonicum 46, M8 and 634b (genetic groups USDA 6, USDA 4, USDA 110, respectively) from the nodules. The use of Rhizohumin based on the composition of B. japonicum strains of different genetic groups allows the correction of rhizobial populations in soil, to form balanced symbiotic systems and increase the soybean yield of varieties Smolyanka and Legend by 17-24% compared to the control. Rhizohumin provided significant increases in soybean yield at the level of 15–28% under production conditions. Experimental and industrial regulation for the production of Rhizohumin based on the B. japonicum 46 + B. japonicum KВ11 strains composition have been developed. Product Description popup.authors Ushakova Marharyta A Shakhovnina Olena O popup.nrat_date 2021-03-17 Close