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Information × Registration Number 0221U100732, 0120U101483 , R & D reports Title Investigation of gene expression of desaturase of cyanobacteria in transgenic tobacco plants to adapt to ionizing and ultraviolet radiation popup.stage_title Head Kyrpa-Nesmiian Tetiana M., Registration Date 08-01-2021 Organization Institute of Cell Biology and Genetic Engineering popup.description2  We used Nicotiana tabacum plants that contain in their genome the desC gene, which encodes the Δ9-acyl-lipid desaturase of the cyanobacterium Synechococcus vulcanus, and the desA gene, which encodes the Δ12-acyl-lipid desaturase of the cyanobacterium Synechocystis sp. PCC 6803. The effect of ionizing radiation in doses of 1g, 5g, 10g was investigated. on the RUM-17 device. The activity of the enzyme superoxide dismutase, the level of accumulation of malonic dialdehyde, the level of electrolyte loss were determined. Analysis of plant resistance to ultraviolet radiation was performed after exposure to OSRAM lamp with a predominant spectrum of C, in doses of 4.36 W / m², 21.8 W / m², 43.6 W / m², 65.4 W / m². The activity of the enzyme superoxide dismutase, the level of MDA accumulation, electrolyte loss and the level of flavonoid growth were studied. It was found that the expression of cyanobacterial desaturase genes does not reduce the resistance of plants to UV and IP. A lower level of MDA accumulation in tobacco plants with cyanobacterial desaturase genes as a marker of unsaturated fatty acid oxidation and a lower level of electrolyte loss after exposure to stress of both species were studied. An increase in the proportion of unsaturated fatty acids in all experimental plants after UV and IR is shown. Research methods - spectrophotometric method, biochemical, gas chromatography. Product Description popup.authors Potrohov Andrij O popup.nrat_date 2021-03-17 Close