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Information × Registration Number 0221U100866, 0120U104870 , R & D reports Title Effects of stress factors on prion-like proteins synthesis in plants popup.stage_title Head Rashydov Namik М., Registration Date 10-01-2021 Organization Institute of Cell Biology and Genetic Engineering of the National Academy of Sciences of Ukraine popup.description2  The analysis was carried out and the experimental approaches for the determination of proteins with α- and β-conformation in leaves and seeds of plants after the action of ionizing and UV radiation, salinization, drought and introduction conditions by the method of FTIR-spectroscopy were substantiated. A database of FTIR-spectra of plants - arabidopsis, flax, soybean, poplar, parrotia, etc. - was analyzed and created. Primers of a number of plants genes were analyzed and selected for evaluation of their expression by qPCR method. FTIR spectroscopy data about the protein domains of amide I and amide II, which consist in the epidermis of arabidopsis leaves and soybean and flax seeds under chronic irradiation and in the leaves of arabidopsis, poplar and parrotia under different types of stress. The obtained results show the increasing of the level of β-structures in proteins under the influence of stress factors, that possibly allow the appearance of prion-like properties in proteins. Product Description popup.authors popup.nrat_date 2021-01-12 Close