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Information × Registration Number 0221U100906, 0120U105315 , R & D reports Title To determine the effect of pesticides HCG and HCB on the growth and development of agricultural plants in vitro popup.stage_title Head Yemets Alla I., Доктор біологічних наук Registration Date 11-01-2021 Organization Institute of Food Biotechnology and Genomics of the National Academy of Sciences of Ukraine popup.description2  The effects of hexachlorobenzene (HCB) and hexachlorocyclohexane (HCCG) on tomato (Lycopersicon esculentum Mill) seed germination and induction of morphogenesis in in vitro culture of L. esculentum cultivar Lagidny and wheat (Triticum aestivum L) were studied. It has been established that HCB in concentrations of 1, 2.5 and 5 mg /L does not significantly affect the process of germination of L. esculentum seeds. The efficiency of seed germination was almost 100%, as in the control. After adding 10 mg /LHCB to the nutrient medium MS, the germination efficiency decreased by an average of 15%. Instead, a study of the effect of HCB on the induction of morphogenesis in culture of isolated cells and plant tissues of L. esculentum in vitro showed that the addition of 5 mg/L of HCB slowed the formation of shoots, and at 10 mg/L completely inhibited the shoot regeneration in vitro, also observed dose-dependent inhibition or lack of callus formation on the surface of the explants. To study morphogenetic potential in the culture of wheat explants, HCB was used at a concentration of 1.5 and 3 mg/L. As a result of the conducted researches it was established that callus formation on wheat explants is observed on the 7th-8th day on the medium without HCB. On media containig HCB, solvents hexadecane and acetone, the process of callus formation was slower, and on some explants (mature embryos) the appearance of necrotic cells was observed. Delayed or no shoot formation was observed compared to standard control, indicating an extremely toxic effect of HCB on growth and morphogenetic processes in in vitro wheat culture. The effect of HCCG on seed germination and morphogenetic processes in in vitro culture of L. esculentum and T. aestivum L. was also studied, and it was found that this compound causes comparable effects with HCB. Product Description popup.authors Yemets Alla I. Shysha Elena M. popup.nrat_date 2021-01-11 Close