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Information × Registration Number 0221U100935, 0116U003073 , R & D reports Title To investigate the conversion features of the diazotrophs to dormant under the actions of the substances of different chemical composition popup.stage_title Head Kozar Serhii F., Доктор сільськогосподарських наук Registration Date 11-01-2021 Organization Institute of Agricultural Microbiology and Agro-industrial Manufacture of National Academy of Agrarian Sciences popup.description2 The object of study - active strains of diazotrophs, polysaccharide-protein complex, functional activity of bacteria after resting. The aim of the work is to study the viability of nitrogen-fixing bacteria of different species at rest with the formation of specific and nonspecific forms under the action of substances of different chemical composition, as well as the functional activity of diazotrophs after resting. Research methods - laboratory (cultural and morphological; deep cultivation; liquid phase fermentation; light microscopy; biochemical and physiological tests; gas chromatography); vegetation and field; mathematical and statistical. The influence of polymer additives, carbohydrates, polyhydric alcohols and protein substances on the transition of bacteria of the genera Azospirillum, Bradyrhizobium, Rhizobium, Azotobacter to a state of rest and preservation of their viability has been determined. It was found that the largest number of viable cells of the studied bacteria was with the use of polysaccharide-protein complex (PBC). The nitrogenase activity of bacteria, their ability to synthesize substances of phytohormonal nature and, accordingly, the growth-stimulating effect on cultivated plants after long-term storage remains high. The influence of PBC on the functional activity of nitrogen - fixing bacteria of different species during their introduction into agrocenoses of agricultural crops has been studied. It is established that application of the investigated complex allows to carry out premature bacterization of seeds. Based on the results of the performed research, a method of preserving the viability and functional activity of diazotrophs at rest has been developed. The introduction of the developed method makes it possible to prematurely bacterize the seeds of agricultural crops, which increases the manufacturability of microbial preparations in agriculture.  Product Description popup.authors Yevtushenko Tetiana A Bilokonska Oksana M Usmanova Tetiana O. popup.nrat_date 2021-01-11 Close