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Information × Registration Number 0221U101222, 0119U101455 , R & D reports Title Ion and membrane mechanisms of regulation of calcium homeostasis and myometrial contraction activity at different stages of pregnancy popup.stage_title Head Kosterin Serhii O., Доктор біологічних наук Registration Date 18-01-2021 Organization Department for special training National academy of science of Ukraine at Kiev University popup.description2  The research have examined changes in the contractility of the myometrium at term under conditions of mechanical stress of varying degrees (10%, 25% tissue stretching), which indirectly affects the activity of enzymes involved in the synthesis of arachidonic acid derivatives, in particular epoxyeicosatric acid which may modulate the functioning of TRPC4 ion channels. It was found that the contractile response of a cell varies depending on the degree of its mechanical stretching, and the action of oxytocin and TRPC4 ion channel agonists differs from that without mechanical stress. These data are significant in the context of the use of ion channel agonists as pharmacological means of normalizing the function of the myometrium. The study developed an algorithm for quantitative analysis of uterine contractions, which consists in the use of templates for automatic search of peaks Amplitude, halfwidth, frequency, area under the curve and time constants of rise and decay are suggested to be determined, as well as the contribution of these parameters to the work produced by the muscle. It was found that the calix[4]aren C-1087, the inhibitor of Ca2+,Mg2+-ATPase activity of myometrium cells plasma membrane, stimulates the increase of Ca2+ concentration in the smooth muscle cells cytoplasm, which does not depend on the state of pregnancy. These data are the basis for further studies of calix[4]arens as selective inhibitors of Ca2+,Mg2+-ATPase activity of myometrium cells plasma membrane, as well as for the development of pharmacological uterotonic agents on their basis for correcting the contractile function of the uterus at pathological conditions. The ATP concentration-dependent increase of ionized calcium concentration in mitochondria matrix in the absence of exogenous Са2+ was registered. Several evaluations were made, thus the Hill coefficient equals 3,18 ± 0,27 and activation constant for ATP equals 0,97 ± 0,07 mM. This process does not involve PTP formation or H+- Product Description popup.authors Babich Lidiya G. Veklich Tetyana O Danilovich Ganna V. Danilovich Yuriy V. Zholos Oleksandr V. Kosterin Sergiy О. Shlikov Sergiy G. popup.nrat_date 2021-01-18 Close