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Information × Registration Number 0221U101265, 0120U103333 , R & D reports Title Development of cardiomyogenic fibroblast reprogramming technology using CRISPR-Cas9 system as a potential method of improving heart function after heart attack popup.stage_title Head Piven Oksana O., Кандидат біологічних наук Registration Date 18-01-2021 Organization Institute of Molecular Biology and Genetics of NAS of Ukraine popup.description2  During the year, bioinformatics analysis of promoter regions of human cardiogenesis genes was performed: GATA4, MEF2C, TBX5, HAND2 MYOD1. Designs of promising gRNAs for activation of human cardiogenesis genes by CRISPR-VPR system have been developed. All sequences were synthesized and cloned into a plasmid for Cas9sgRNA expression (Addgene # 68463). A plasmid expressing fusion proteins of catalytically inactivated Cas9 and transcription activation domains - VPR was also developed. Therefore, a collection of plasmids expressing selected gRNA sequences was created for further molecular genetic analysis and in vitro validation. In general, the data obtained by us are a necessary and logical basis for studying the prospects of using the CRISPR system for cell reprogramming for therapeutic purposes. Product Description popup.authors Macewicz Larysa L Ruban Tetiana P Sklyarenko Yuliya V popup.nrat_date 2021-01-18 Close