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Information × Registration Number 0221U101367, 0120U104979 , R & D reports Title Differential control of regulatory networks involved in the maintaining of epithelial-mesenchymal plasticity of breast adenocarcinoma cells popup.stage_title Head Filonenko Valerii V., д.б.н. Registration Date 20-01-2021 Organization Institute of Molecular Biology and Genetics of NAS of Ukraine popup.description2  Malignant neoplasms of the breast are quite common in Ukraine and around the world and make up a significant proportion of malignant human tumors. Usually, anticancer therapy is based on the suppression of factors found in overexpressed or over-activated form compared to normal tissue. In the case of differentiated breast tumors, it is primarily about receptors for sex hormones, growth factors and intracellular signaling molecules. Often, the combined suppression of several targets increases the effectiveness of anticancer therapy. One of the overactivated and overexpressed signaling cascades in breast carcinomas is the S6K signaling pathway. In a number of clinical studies, the signaling pathway and S6K proper act as targets for anticancer therapy. This is due to their involvement in the regulation of cell proliferation and migration on the one hand, and the ability of ligands to independently activate the estrogen receptor kinase by the ribosomal protein S6 on the other. However, given the existence of a number of S6K isoforms, understanding the function of each and, if necessary, finding / developing specific inhibitors for them seems to be an urgent task. During the project, MCF-7 cell lines with differential expression of S6K1 isoforms achieved by genomic editing using CRISPR technology were characterized. The influence of differential expression of isoforms S6K1 - p60, p70, p85 on the state of PI3K-dependent signaling cascade by the level of activation (phosphorylation) of key components of the signaling pathway is estimated. Peculiarities of S6K1 isoforms activation and their subcellular localization have been studied. Product Description popup.authors Bdzola Anna V Hotsuliak Nazar Ya. Khoruzenko Antonina I popup.nrat_date 2021-01-20 Close