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Information × Registration Number 0221U101636, 0116U001400 , R & D reports Title To study the physiological and biochemical characteristics of male germ cells by using biologically active substances in polymeric carriers. popup.stage_title Head Sharan Mykola M, Registration Date 23-01-2021 Organization Institute of Animal Biology of NAAS popup.description2 Qualitative indicators of animal sperm at different stages of cryopreservation under the action of optimal doses of nanocompounds based on nanoaquacitrates and nanoaquasuccinates Mn, Cu, Zn as part of media for cryopreservation were studied. Optimal doses of nanoaquacitrates and nanoaquasuccinates of Cu, Mn and Zn and polymers of nanocompounds in male ejaculates diluted with industrial diluents were established. The optimal concentrations at which the intensity of oxidative processes in the diluted semen of bulls is normalized are: 0.01 mg / l Mn-, 0.004 mg / l Cu- and 0.06 mg / l Zn-nanocitrates 0.05 mg / Mn-, 0.005 mg / l Cu- and 0.05 mg / l Zn-nanosuccinates. Similar concentrations of nanocitrates and nanosuccinates when added to rams semen. At these optimal concentrations of nanocompounds, the content of total protein, respiratory activity of sperm, activity of enzymes-markers of fertility - succinate dehydrogenase (SDG) and cytochrome oxidase (CO) had the highest values. The optimal doses of nanocitrates and nanosuccinates Zn, Mn, Cu in the diluents of ejaculate of bulls and rams, which provide the highest indicators of sperm quality after equilibration and deconservation: sperm activity, their dynamic parameters and survival; respiratory activity of sperm; activity of SDG and CO. A methodology for creating environments for cryopreservation of sperm of breeding animals of productive animals based on the use of metal nanocompounds has been developed. Media for cryopreservation of semen of bulls and rams, which contain nanoaquacitrates and nanoaquasuccinates Mn, Cu, Zn, provide an increase in the fertilizing ability of sperm by 10-15%. Advantages over the analogue: by reducing the dose of BAS and increasing their effectiveness. Economic efficiency: for 1 UAH of expenses - 1,5-2 UAH of profit. Product Description popup.authors Andrushko Oleksandr B Bodnar Yulya V Grymak Khrystyna M Korbetskyy Andriy R Korniat Serhii B Kuzmina Nataliia V Ostapiv Dmytro D Petruh Iryna M Tatomyr Zinovii V Yaremchuk Iryna M popup.nrat_date 2021-01-23 Close