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Information × Registration Number 0221U101800, 0116U000670 , R & D reports Title Improving of biotechnological methods of basic material and wheat lines with certain agronomical characteristics, adapted to the zonal agro-climatic conditions, obteining popup.stage_title Head Zambriborshch Iryna S, Registration Date 25-01-2021 Organization Selection-Genetic Institute - National Center of Seed and Selection Studies of Ukrainian Academy of Agrarian Sciences popup.description2  The ability to androgenesis in vitro of 192 genotypes of soft winter wheat and 44 genotypes of durum wheat were studied. 457 green regenerants of T. aestivum L. were obtained. Two genotypes of winter soft wheat (№120, №132) were identified and may be recommended as haploproduction donors The most optimal combination of the induction nutrient medium composition for the cultivation of T. durum anthers was revealed: mineral and organic composition of C17B medium with the addition of 0.75 mg/l BAP, 0.25 mg/l kinetin, 0.25 mg/l NAA, sucrose (60 g/l) and glucose (17.6 g/l). After the stages of soil adaptation, vernalization and rearing in artificial climate conditions, the seeds of 137 digaploid lines of soft winter wheat (30% of the obtained regenerating plants) were transferred to the Department of Wheat Breeding and Seed Production of SGI-NCSSVS for further research. Obtained patents for a utility model: UA 108512 U "Nutrient medium C17B for the induction of tumors in the culture of anthers of durum wheat in vitro". № u201512315 dated 25.07.2016; "Selection of donor plants to increase the efficiency of biotechnology for the production of linear material of soft winter wheat in the early stages of selection". № u 2020 05779 dated 07.09.2020 Product Description popup.authors Chekalova Mariya S Chekalova Mariya S Shestopal Oksana L Shestopal Oksana L popup.nrat_date 2021-01-25 Close