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Information × Registration Number 0221U101882, 0118U005469 , R & D reports Title The effect of glucose metabolism on anoikis and metastatic potential of cancer cells popup.stage_title Head Solianyk Galyna I., Доктор фізико-математичних наук Registration Date 26-01-2021 Organization R.E. Kavetsky Institute of Experimental Pathology, Oncology and Radiobiology National Academy of Sciences of Ukraine popup.description2  For the first time, comparative studies of tumor cell resistance with different metastatic potential to anoikis (apoptotic cell death under de-adhesion from the extracellular matrix), which is known to ensure the survival of metastatic cells in the first stage of the metastatic cascade. It has been shown in vitro that the detachment of tumor cells from the adhesive surface is accompanied by a decrease in glycolysis intensity (expressed in a reduction of glucose consumption and lactate production), a significant increase of intracellular ROS and the percentage of apoptotic cells, statistically significantly higher in tumor cells with low metastatic potential. Inhibition of lactate dehydrogenase activity (as a promising target for therapeutic intervention) with oxamate, which promotes both intracellular ROS levels and induction of apoptosis, causes a cytotoxic effect, more pronounced for cells with low metastatic potential. Meanwhile, oxamate administration to animals with inoculated highly metastatic Lewis lung carcinoma cells showed no antitumor and antimetastatic effect in vivo, in particular due to oxamate ability to stimulate tumor angiogenesis indirectly by increasing the proportion of tumor-associated macrophages with proangiogenic properties of M2 phenotype. Product Description popup.authors Horbach Oleksandr I. Kolesnyk Denys L. Pyaskovska Olga M. Sviatetska Vitalina M. Solianyk Galyna I. Khaietskyi Igor K. Shliahtova Nataliia A. Yurchenko Olga V. popup.nrat_date 2021-01-26 Close