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Information × Registration Number 0221U102081, 0118U003742 , R & D reports Title Development of the concept of regulation of plants growth and stress resistance for their adaptation to the conditions of space flights by attracting cellular and biological resources popup.stage_title Head Blume Yaroslav B., Доктор біологічних наук Registration Date 28-01-2021 Organization Institute of Food Biotechnology and Genomics of the National Academy of Sciences of Ukraine popup.description2  Within the scope of the research for this project the study of morphological, molecular genetic and cytological analysis for the radiation induced cell death types features development in plants grown under altered gravity conditions was conducted. With the use of Pisum sativum as one of the model objects it was established that irradiation causes dose-depended growth inhibition of week-old seedlings dry mass, chromosomal aberrations frequency increase and with the 4 and 6 Gy dose interrupts structural homeostasis of root apical meristem and inhibits the development of lateral roots. In this research Arabidopsis thaliana (L.) plantlets general condition in regards of roots basic morphological features and anatomical structure of both control and stress influenced plants was also evaluated. It was shown that A. thaliana plantlets general condition at clinostatting was similar to control. Clinostatting plantlets differed in growth disorientation due to the constant change of their position relative to the gravitation vector. Root apex sizes and anatomic features after clinostatting were similar to control plants. Slight differences in width of the elongation zone cells that had no significant influence on general root structure. It was also shown that after 2 Gy irradiation the morphology of plantlets was similar to control. The study of microtubules of A. thalina (GFP-MAP4) plantlets showed reorganization of cortical microtubules in the root transition zone after the stress influence. As microgravity and radiation exposure are stressors that lead to cell and morpho-physiological changes in plants the study of its influence on autophagy development in A. thalina roots was conducted out. The results of transcriptional analysis of nine atg8 genes activity results indicates that both autophagy development under the influence of microgravity, and that a part of these genes could be considered as more specific one for autophagy full implementation under experimental condi Product Description popup.authors Yemets Alla I. Blume Yaroslav B. Horiunova Inna I. Kravets Helen A Plokhovska Svitlana H. Shadrina Ruslana Yu. popup.nrat_date 2021-01-28 Close